Search results for "Enological aptitude"

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Yeast ecology of vineyards within Marsala wine area (western Sicily) in two consecutive vintages and selection of autochthonous Saccharomyces cerevis…

2012

In this work, the yeast ecology associated with the spontaneous fermentation of Grillo cultivar grapes from 10 vineyards was analyzed from grape harvest till complete consumption of must sugars. The microbiological investigation started with the plate count onto two culture media to distinguish total yeasts (TY) and presumptive Saccharomyces (PS). Yeasts were randomly isolated and identified by a combined genotypic approach consisting of restriction fragment length polymorphism (RFLP) of 5.8S rRNA gene and 26S rRNA and sequencing of D1/D2 domain of the 26S rRNA gene, which resulted in the recognition of 14 species belonging to 10 genera. The distribution of the yeasts within the vineyards s…

IdentificationGenotypeSaccharomyces cerevisiaeAcetic Acid; Culture Media; DNA Fungal; Ethanol; Fermentation; Genotype; Hydrogen Sulfide; Microsatellite Repeats; Polymerase Chain Reaction; Polymorphism Restriction Fragment Length; RNA Ribosomal; Saccharomyces cerevisiae; Sicily; Sulfites; Temperature; Vitis; WineBioengineeringWineSaccharomyces cerevisiaeBiologyApplied Microbiology and BiotechnologySaccharomycesPolymerase Chain ReactionEnological aptitudeYeastsGenotypeSulfitesVitisHydrogen SulfidePolymorphismDNA FungalSicilyAcetic AcidRibosomalWineEthanolEcologyIdentification; Enological aptitudes; Saccharomyces cerevisiae; Spontaneous wine fermentation; YeastsTemperatureDNARibosomal RNASpontaneous wine fermentationbiology.organism_classificationYeastCulture MediaFungalRestriction Fragment LengthRNA RibosomalFermentationRNAFermentationRestriction fragment length polymorphismPolymorphism Restriction Fragment LengthBiotechnologySettore AGR/16 - Microbiologia AgrariaMicrosatellite RepeatsJournal of bioscience and bioengineering
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